Kiến thức

HRP polymer Goat Anti-Mouse (IgG) secondary antibody (ab214879)

Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)

    Key features and details

    • Goat Anti-Mouse IgG H&L (HRP polymer)
    • Conjugation: HRP polymer
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Immunoelectrophoresis, IHC-P

    Conjugates logo Related conjugates and formulations

    Agarose

    Alexa Fluor® 405

    Alexa Fluor® 488

    Alexa Fluor® 555

    Alexa Fluor® 568

    Alexa Fluor® 594

    Alexa Fluor® 647

    Alexa Fluor® 680

    Alexa Fluor® 750

    Alexa Fluor® 790

    Alkaline Phosphatase

    APC

    beta-galactosidase

    Biotin

    Cy2 ®

    Cy3 ®

    Cy3.5 ®

    Cy5 ®

    Cy5.5 ®

    DyLight® 488

    DyLight® 550

    DyLight® 594

    DyLight® 650

    FITC

    Glucose Oxidase

    Gold 10nm

    Gold 20nm

    Gold 6nm

    HRP

    IRDye® 680RD

    IRDye® 800CW

    PE

    Texas Red ®

    TRITC

    Unconjugated

    Unconjugated

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    Overview

    • Product name

      Goat Anti-Mouse IgG H&L (HRP polymer)

      See all IgG secondary antibodies

    • Host species

      Goat
    • Xem thêm: Định Nghĩa-Công Suất Tổ Máy Phát Điện. – CÔNG TY TNHH KỸ THUẬT-ĐIỀU KHIỂN DƯƠNG PHÁT.

      Target species

      Mouse
    • Specificity

      Based on IEP, ab214879 reacts with heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins. No cross-reactivity is observed to non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig or rabbit.
    • Tested applications

      Suitable for: Immunoelectrophoresis, IHC-P

      more details

    • Immunogen

      Other Immunogen Type corresponding to Mouse IgG. (Purified Mouse IgG, whole molecule).

    • Conjugation

      HRP polymer

    Properties

    Applications

    The Abpromise guarantee

    Our

    Abpromise guarantee

    covers the use of ab214879 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Immunoelectrophoresis
    Use at an assay dependent concentration.
    IHC-P

    (1)

    Use at an assay dependent concentration.

    This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour.

    Notes
    Immunoelectrophoresis
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.

    This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour.

    Images

    • Immunohistochemical analysis of Triple positive breast cancer tissue stained with Ki-67 and detected with ab214879.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (6)

    ab214879 has been referenced in 6 publications.

    • Kobayashi H  et al. The Balance of Stromal BMP Signaling Mediated by GREM1 and ISLR Drives Colorectal Carcinogenesis. Gastroenterology 160:1224-1239.e30 (2021).

      PubMed: 33197448

    • Lucafò M  et al. miR-331-3p is involved in glucocorticoid resistance reversion by rapamycin through suppression of the MAPK signaling pathway. Cancer Chemother Pharmacol 86:361-374 (2020).

      PubMed: 32776229

    • Zarbakhsh S  et al. Irisin protects the substantia nigra dopaminergic neurons in the rat model of Parkinson’s disease. Iran J Basic Med Sci 22:722-728 (2019).

      PubMed: 32373292

    • Zhang C  et al. FOXA1 is upregulated in glioma and promotes proliferation as well as cell cycle through regulation of cyclin D1 expression. Cancer Manag Res 10:3283-3293 (2018).

      PubMed: 30233244

    • Li F  et al. Blocking the CD47-SIRPa axis by delivery of anti-CD47 antibody induces antitumor effects in glioma and glioma stem cells. Oncoimmunology 7:e1391973 (2018).

      PubMed: 29308321

    • Fang L  et al. Resistance to epithelial growth factor receptor tyrosine kinase inhibitors in a patient with transformation from lung adenocarcinoma to small cell lung cancer: A case report. Oncol Lett 14:593-598 (2017).

      PubMed: 28693210

    Customer reviews and Q&As

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    12 of 2 Abreviews or Q&A

    Viewing: 10Viewing: 50Viewing: 100Sort by: Highest votesSort by: Lowest votesSort by: Newest FirstSort by: Oldest First

    Goat anti-mouse IgG (HRP polymer) works well for FFPE human sample

    Good

    Abreviews

    Abreviews

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    In this study to test how well the goat anti-mouse IgG (HRP polymer) works on FFPE human tissue sections, the FFPE human skin tumor section was deparaffinized and boiled in antigen retrieval buffer, 10mM Sodium Citrate pH6. Overnight incubation in a mouse anti-Smooth muscle actin primary antibody from Sigma Aldrich. The goat anti-mouse IgG (HRP polymer) secondary antibody is used at 1:1 dilution in 2% BSA for 90 minutes at room temperature. The slides were then developed in DAB solution and counter-stained with hematoxylin.
    The staining works well.

    Abcam user community

    Verified customer

    Submitted Jul 30 2018

    Goat anti-mouse IgG (HRP polymer) works well in pig tissue

    Excellent

    Abreviews

    Abreviews

    Application
    Immunohistochemistry paraffin embedded sections

    In this study to test how well the goat anti-mouse IgG (HRP polymer) works on FFPE pig tissue sections, the FFPE human pig lung section was deparaffinized and boiled in antigen retrieval buffer, 10mM Sodium Citrate pH6. Overnight incubation in a mouse anti-Smooth muscle actin primary antibody from Sigma Aldrich. The goat anti-mouse IgG (HRP polymer) secondary antibody is used at 1:1 dilution in 2% BSA for 90 minutes at room temperature. The slides were then developed in DAB solution and counter-stained with hematoxylin.

    The staining works well. The picture enclosed shows clear SMA staining in pig lung and a negative control insert is included for reference.

    Abcam user community

    Verified customer

    Submitted Jul 30 2018

    Chuyên mục: Kiến thức

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